The HPLC (BioCat 700E system from Applied Biosystems, CA, USA) comparison with the MALDI-ToF MS data was performed preparing the samples as for the peptide adsorption experi-
up to 100 ±2.2, above 100 to 150 ±2.8, above 150 to 275 ±3.4, above 275 to 425 ±4.1, above 425 ±4.8 (1)
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This solution was stored in a brown glass bottle and kept at 4 °C. Under these conditions it was stable for at least 7 days. Fresh working solutions were prepared daily by appropriate dilution of the stock solutions. The HPLC eluent was a solution of methanol (40%, v/v) in phosphate buffer (pH 5.50, 0.020 M).
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The residue was redissolved in 100 μL of HPLC mobile phase and 2 μL was injected into the HPLC–MS-MS system. Method validation Since glutamine is an endogenous compound, the endogenous interference exists when using chromatographic assay.
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The ethanol fraction was collected in glass vials filled with dry cotton (105 °C for 24 h) and the ethanol contained in the gaseous CO 2 stream was measured by an alcoholmeter device (BFD 100 digital, Instrutherm, São Paulo, Brazil) at determined time intervals (10, 20, 30, 60, 90 and 120 min). The drying kinetics were performed for 2 h and
2001 1 7.5. 2001 1 7.5. 2001 1 7.5. 2001 1 7.5. 1998 6. 1998 6.5. 2004 9.5. 2004 9.5. 2008 3 7.5. 2008 3 7.5. 2009 9.5. 2009 1 9.5. 2009 1 9.5. 1953 1 9.5. 2003 1 9