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Jun 13, 2017 · Ultra-High Performance Liquid Chromatography (UHPLC) It is technology based on the principle that smaller size particles lead to higher efficiency, faster separations with superior resolution, and sensitivity. However, to tolerate extreme pressure from particles smaller than 2 µm, the system needs to be able to handle high backpressure.

UPLC operates at considerably high pressures of 15,000 psi (psi, in this case, stands for Pounds per square inch and is a unit of pressure), while HPLC operates at relatively lower pressures of 500-6000 psi. The key difference between HPLC and UPLC here is that UPLC has a clear advantage of HPLC, thanks to its higher pressure.

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•Normal flow rates in HPLC are in the 1- to 2-mL/min range. •Typical pumps can reach pressures in the range of 6000-9000 psi (400- to 600-bar). • During the chromatographic experiment, a pump can deliver a constant mobile phase composition

Oct 10, 2013 · The role of the pump is to propel (force) a liquid (the mobile phase) through the chromatograph at a specific flow rate, expressed in ml/min. Normal flow rates in HPLC are 1-2 ml/min and typical pumps can reach pressures in the range of 2000 – 9000 psi but in applications covered under UHPLC mode operating pressure can be as high as 15000-18000 psi.

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Aijiren Technologies, one of the leading technology companies for measurement engineering, offers a broad array of innovative analytical HPLC columns. Analytics-Shop offers the whole range of Aijiren HPLC columns: Material: Zorbax, StableBond, Extend, Poroshell, Polaris, Pursuit, ChromSpher, etc.

Electrochemical detection (ECD) for HPLC is an extremely selective and sensitive detection technique that is applied in a number of analyses such as the neurotransmitters dopamine, serotonin and noradrenalin. In combination with the proper electronics, ECD has a linear dynamic range of more then 6 orders of magnitude.

To ensure the stability and performance of chromatography and spectroscopy analyses, Aijiren offers a range of laboratory supplies to meet the challenges of any application. Our extensive portfolio includes gas management products, including gas purifiers, tubing, traps, flow meters, and leak detectors.

HPLC Solvent Management Waters HPLC pumps enable you to customize HPLC Systems to accommodate your solvent delivery needs, whether for isocratic or gradient, analytical or preparative. HPLC Sample Management For HPLC sample aspiration, automated and manual injection, and fraction collection, Waters provides a range of sample management options.

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ceuticals using high-performance liquid chromatography (HPLC) and covers the literature up to the end of 2003. The current volume is a continuation of HPLC Methods for Pharmaceutical Analysis, published in four volumes from 1997 to 2000. The previous volumes described methods published in the literature through the middle of 1998.

All HPLC solvents should be filtered through a 0.45 µm filter before use. This removes any particulate matter that may cause blockages. After filtration, the solvents should be stored in a covered reservoir to prevent contamination with dust, etc Filtering HPLC solvents will benefit both your chromatography and HPLC system.