Feb 5, 2015 · Choosing certified autosampler vials can significantly reduce the opportunity for such issues to affect analysis. For highly sensitive samples such as pesticides or semi volatiles, as well as samples that are prone to sudden shifts in pH, deactivated vials are best, because their surface is more hydrophobic and inert.
The peptide could be sticking to any surface it comes in contact with, including pipette tips, sample storage tubes, or autosampler vials. Switching suppliers or materials may help. Take a fresh look at your sample prep protocol – could a particularly hydrophobic peptide be precipitating?
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Peptide analysis by LC-HRAM-MS offers orthogonal solutions for detection and monitoring of HCPs compared to immunological methods with the unbiased discovery of HCP impurities and subsequent quantitation. HRAM MS data combined with Thermo Scientific BioPharma Finder 4.1 software provides comprehensive HCP qualitative and quantitative analysis.
vials 13-425 Screw Thread vials and 4 mL Shell vials 20 mm Headspace Crimp vials Crimp vials 20 mm Headspace Crimp and 18 mm Screw Thread vials 24-400 Screw vials (EPA) Well Plates Manufacturer Model 7 mm OD 8 mm OD 6 mm OD 12 mm OD 12 mm OD 12 mm OD 12 mm OD 15 mm OD 22 mm OD 23mm OD 22.5-23 mm OD 28 mm OD -
Guidelines for sample preparation: Use appropriate solvents for the ESI-MS system. a. Appropriate solvents include: • Acetonitrile. • Methanol. • Water. b. If your compound is not sufficiently soluble in one of the above, you can first dissolve it in DMSO, but you must dilute the DMSO to 0.1%.
CHAT NOW. 2mL 9-425 Amber Screw Top Autosampler Vials w - FiltrousType: Screw Top ; Diameter: 9mm ; Color: Amber ; Qty: 100/unit ; Price per Piece: $0.36.2 mL Screw Top Vials and Screw Cap.
Overview. For large molecule LC-MS bioanalysts who need the ultimate sensitivity, reproducibility and sample stability over multiple analyses. Improve detection limits and analysis reproducibility for peptide and protein quantification with these low adsorption plates and vials.
Jun 27, 2023 · In general, modern chromatography liquid autosamplers accept 12 x 32mm vials, so we will assume that a 12 x 32mm vial is compatible with your autosampler (Headspace autosamplers accept 20mm wide
Picking the right mobile phases can be key to developing a robust method – but good mobile phase hygiene is just as important! Aqueous mobile phases are great breeding grounds for microbes. These microbes can lead to clogging at any point of the flow path, from pump chambers to capillaries to LC columns.
HPLC & GC Certified Screw Vial & Cap Kits for 2 mL Samples. Precleaned, EPA certified assembled kits; includes 40 mL screw vials and 24 mm. open top screw caps. 0.3 and 1.5 mL screw top vial-cap kits for analysis of small sample volumes. For use with environmental analyses under EPA regulations.
The analysis in GC starts with the introduction of the sample onto the column. It is also by far the most critical step in the entire analysis. The liquid sample is vaporized either inside the column itself or in a vaporization chamber positioned just in front of the column. The injection system should fulfill the following requirements:
These vials have a thicker glass wall to withstand higher temperature and internal pressure for the analysis of volatile gas in the vial’s headspace. Headspace vials with 20 mm crimp tops and 18 mm screw tops in sizes ranging from 6 mL to 20 mL are available in clear or amber glass.
TruView Vials exhibit the lowest surface adsorption of any LC autosampler vial on the market. 96-Well Plate, 700 µL per wellis made from a clean grade of polypropylene. The wells are designed to maximize sample recovery for all ACQUITY UPLC®Systems.
Pump Right was used for the SEC analysis and ran isocratically with 10 mM ammonium acetate, pH 6.7 with 5% acetonitrile. Pump Left was used for the reversed phase chromatographic analysis. The system configuration is shown in Figure 2. Figure 2. Schematic of 2D LC system configuration Table 2. Timing of the gradient and loop fill