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May 03, 2019 · Each Chl-a vial contained 1 mg of pure chlorophyll, in which each vial’s content was dissolved in 50 mL of high-performance liquid chromatography (HPLC) grade (90% acetone) to give 20 mg/L stock solutions, which were further diluted when needed in 90% acetone to give solutions of 0.2 µg L −1.
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